Development of Site-specific Artificial Ribonucleases
نویسندگان
چکیده
منابع مشابه
Development of Peptide Nucleic Acid Based Artificial Ribonucleases PNAzymes
Since mRNA plays such a vital role in the transfer of genetic code from DNA to proteins, approaches involving interference with mRNA are of great interest for use in therapeutics. Amongst these, peptide nucleic acid based nuclease systems (PNAzymes) are being developed for in vivo gene silencing in future. The PNAzymes hybridize to the RNA, bringing a catalytic group to the phosphodiester bond ...
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Design of site-selective artificial ribonucleases (aRNases) is one of the most challenging tasks in RNA targeting. Here, we designed and studied oligonucleotide-based aRNases containing multiple imidazole residues in the catalytic part and systematically varied structure of cleaving constructs. We demonstrated that the ribonuclease activity of the conjugates is strongly affected by the number o...
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Lanthanide complexes covalently attached to oligonucleotides have been shown to cleave RNA in a sequence-specific manner. Efficient cleavage, however, is at present limited to single-stranded RNA regions, as RNA in a duplex is considerably more resistant to strand scission. To overcome this limitation, we have designed and synthesised artificial nucleases comprising lanthanide complexes covalen...
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Chemically modified nucleotide monomers were incorporated into adjacent terminal positions of two separate oligonucleotides complementary to an RNA target; all possible combinations of the catalytic units were tested, resulting in an artificial nuclease that showed high activity and catalytic turnover.
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2007
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/nrm102